Design, Synthesis, and Evaluation of Synthetic Particulate Delivery Systems in DNA and Protein Vaccine Delivery

Design, Synthesis, and Evaluation of Synthetic Particulate Delivery Systems in DNA and Protein Vaccine Delivery

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The overall goal of this project was to engineer synthetic polymer based delivery systems to enhance the potency of protein and DNA vaccines. We have synthesized hybrid polymeric PLGA microparticles; surface modified by chemical conjugation of polycations such as branched and linear poly (ethyleneimine) PEI and with solvent free Atmospheric Plasma Glow discharge (APG) based approaches. Extensive characterization demonstrates that our formulations: (a) have excellent reproducibility, (b) are efficiently taken up by phagocytic cells, (c) have endo/phagosomal escape facilitating properties, (d) are non toxic to cells, and (e) have improved transfection efficiencies at early time points in phagocytic cells in vitro. We have also demonstrated that these formulations considerably enhance potency of cancer genetic vaccines without the use of known immune potentiators in mice models in vivo. The potential to include known soluble adjuvants in our design can have far reaching effects in realizing combinatorial vaccine and adjuvant delivery systems for cures in cancer and infectious diseases.The total volume for the protein adsorption process was 1ml in a 1.5ml microcentrifuge tube. ... Post protein adsorption, the microparticles were centrifuged at 4, 000 rpm using a 5810R refrigerated Eppendorf centrifuge ( Eppendorf, Fischer Scientific, USA) for 20 minutes. ... using the BCA assay for protein estimation as per the instructions from Pierce 97 Zeta potential analysis of plasma discharge Proteinanbsp;...


Title:Design, Synthesis, and Evaluation of Synthetic Particulate Delivery Systems in DNA and Protein Vaccine Delivery
Author:
Publisher:ProQuest - 2006
ISBN-13:

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